Study on Optimization, Degreasing and Destaining Potentials of Glycophospholipid Biosurfactant Produced by Bacillus Anthracis S62A

Oil-polluted soil is one of the major sources biosurfactant producing bacteria. This study was to optimization, degreasing and de-staining potentials glycophospholipid produced by B. anthracis strain S62A isolated from spent engine oil-polluted soil. Five (5) bacterial isolates associated with oil – polluted primarily screened for production using emulsification index test, displacement surface tension test. The isolate highest screening test results selected, characterized examined its potential on different oil-stained fabric materials. showed that parameters required maximum yield were 20 g slaughter house waste, 10 rice bran, 1% sodium chloride, 9.0 pH, 4 °C temperature, 10% inoculum 5 days incubation period. biochemical profile revealed extracted identified as glycophospholipid. cleaning efficiency obtained observed possess 78, 72 64 % removal stained polyester, cotton chiffon materials, respectively at mg/mL critical micelles concentration (CMC). Statistically, significant differences (P < 0.05) detected among means all surfactant treatments in comparison their controls two-way ANOVA followed Dunnett Therefore, this suggests could be exploited laundry industries formulation detergents well textile dye biotreatment.